Hemagglutinin-Neuraminidase Gene Sequence-Based Reclassification of Human Parainfluenza Virus 3 Variants
نویسندگان
چکیده
منابع مشابه
Hemagglutinin-neuraminidase gene sequence-based reclassification of human parainfluenza virus 3 variants.
The most comprehensive phylogenetic classification of human parainfluenza virus 3 (HPIV-3) was recently developed [PLoS One 2012;7:e43893]. This classification included three distinct clusters (A, B and C) with subdivision of cluster C into four subclusters (C1-4). In the present report, the classification of HPIV-3 was refined by inclusion of 27 overlooked beside newly characterized Saudi vari...
متن کاملCrystallization of biologically active hemagglutinin-neuraminidase glycoprotein dimers proteolytically cleaved from human parainfluenza virus type 1.
We isolated, purified, and characterized the hemagglutinin-neuraminidase (HN) of human parainfluenza virus type 1, with the ultimate goal of producing crystals suitable for three-dimensional X-ray structure analysis. Pronase was used to cleave the globular head of the HN molecule directly from virus particles, forming HN monomers and dimers. The purified dimers retained neuraminidase and hemads...
متن کاملSequence variation in the haemagglutinin-neuraminidase gene of human parainfluenza virus type 3 isolates in the UK.
The sequence variation in a 934 base-pair region of the gene encoding the haemagglutinin-neuraminidase of five human parainfluenza virus type 3 (HPIV3) isolates was determined together with that of a prototype UK strain. All of the clinical isolates were from the Manchester area of the UK and were obtained in 1990, 1991 and 1993. The gene segment was amplified by the polymerase chain reaction u...
متن کاملNeuraminidase activity and syncytial formation in variants of parainfluenza 3 virus.
By a sensitive fluorometric assay method, we could definitely demonstrate neuraminidase activity for two variants of parainfluenza 3 virus, M and SC, which were previously shown to have no detectable neuraminidase activity. The enzyme activities of these viruses were very similar to each other, showing a much lower catalytic rate, a much higher Km value, and a more acidic pH optimum than those ...
متن کاملAntigenic and Structural Properties of the Hemagglutinin - Neuraminidase Glycoprotein of Human Parainfluenza Virus Type 3 : Sequence Analysis of Variants Selected with Monoclonal Antibodies Which Inhibit Infectivity , Hemagglutination , and Neuraminidase Activities KATHLEEN
The hemagglutinin-neuraminidase (HN) gene sequence was determined for 16 antigenic variants of human parainfluenza virus type 3 (PIV3). The variants were selected by using monoclonal antibodies (MAbs) to the HN protein which inhibit neuraminidase, hemagglutination, or both activities. Each variant had a single-point mutation in the HN gene, coding for a single amino acid substitution in the HN ...
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ژورنال
عنوان ژورنال: Intervirology
سال: 2015
ISSN: 0300-5526,1423-0100
DOI: 10.1159/000369208